The theoretical lectures delivered by key scientists in the field are complemented by the direct utilization of steady state and lifetime fluorescence instrumentation and confocal microscopy for FLIM and FRET applications provided by leading companies. Topics addressed in this course include:
• Basic Definitions and Principles of Fluorescence
• Fluorescence Polarization
• Time-Resolved Fluorescence
• Instrumentation
• Data Manipulation and Data Analysis
• Confocal and Multiphoton Fluorescence Microscopy
• FLIM, Fluorescence Lifetime Imaging
• FCS, Fluorescence Correlation Spectroscopy
• Single Molecule Imaging
• Image processing and Deconvolution Approaches
It is recommended that participants have at least a bachelor’s degree in the life sciences, physical sciences or engineering before attending this course.
The hands-on experimental section has limited space available. Practicals will be performed on steady-state, time-resolved, FCS and FLIM instrumentation with detailed supervision and input by lecturers and ISS engineers.
Date: June 4-6, 2012
Contact:
Samantha Redes, Course Coordinator
coordinator@fluorescence-foundation.org
217-359-8681
For more information on the course
