5TH PRACTICAL SUMMER TRAINING ON
CHROMATOGRAPHY, MOLECULAR BIOLOGY & BIOINFORMATICS (PCMB)
June 04 - 15, 2012
Organized by
School of Bio Sciences and Technology (SBST)
VIT University, Vellore – 632 014, Tamil Nadu
Techniques
Cloning of DNA in bacterial expression vectors: PCR amplification of gene → Gel
purification of DNA → Restriction digestion → Ligation → Bacteria
Transformation
Expression and purification of proteins expressed in bacteria : IPTG induction of
gene expression in BL 21 bacteria → Purification of GST tag protein from bacteria → Analysis of purified protein by SDS PAGE
Chromatography : Gel permeation Chromatography, Ion exchange Chromatography & HPLC
Techniques in Bioinformatics and Biophysics
Enriched mRNA preparation – cDNA synthesis – Semi-quantitative RT-PCR
Real time PCR
Primer design for smart cloning in vectors, primer design for gene expression studies, primer editing, probe design.
purification of DNA → Restriction digestion → Ligation → Bacteria
Transformation
Expression and purification of proteins expressed in bacteria : IPTG induction of
gene expression in BL 21 bacteria → Purification of GST tag protein from bacteria → Analysis of purified protein by SDS PAGE
Chromatography : Gel permeation Chromatography, Ion exchange Chromatography & HPLC
Techniques in Bioinformatics and Biophysics
Enriched mRNA preparation – cDNA synthesis – Semi-quantitative RT-PCR
Real time PCR
Primer design for smart cloning in vectors, primer design for gene expression studies, primer editing, probe design.
Eligibility and Selection Criteria
Students of M. Sc / M.Tech / B.Tech in Biochemistry, Microbiology, Life Sciences, Biotechnology or Research Scholars / Faculty members / industrialists belonging to above field are eligible to apply. Only 40 candidates will be selected by first come first serve basis. The selected candidates will be informed by email on or before May 04, 2012.
Registration Fees (Registration fees includes registration kit and working lunch)
• Rs.6,000 for students and research scholars
• Rs.7,500 for faculty members and industrial persons
• Rs.7,500 for faculty members and industrial persons
Accommodation (Will be arranged on request only in VIT Hostel)
Hostel - Rs.150 per day
Registration
Send the registration form along with the prescribed fee as a DD (draft in favour of VIT University, payable at Vellore) to the Organizing Secretary on or before May 4, 2012.
